The application of the rhizome to the study of organization is examined. A use of the rhizome which reflects its expansive and ephemeral nature, rather than one which forecloses its conceptual possibilities, is promoted. This nature is examined in relation to its development from Deleuzian concepts of desire and virtuality. Examples of the use of the rhizome in the study of organization are analyzed for the conceptual potential that they offer and critiqued where they close off this potential. It is suggested that an interplay – between the use and appropriation of the rhizome in the study of organization and the building of rhizomatic ontologies of flow – is desirable for maintaining the rhizome as an open and useful concept.